Insulin Radioimmunoassay Using “INSULIN KIT”
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of insulin in serum and plasma.
Paired serum and plasma samples from subjects were analyzed to determine if there was a difference in the insulin content of serum and plasma. Although there was no difference in the serum and plasma insulin concentrations in the samples as a group, there were differences in the serum and plasma insulin concentrations in individual subjects. Recovery of radioimmunoassayable insulin from clotted...
متن کاملHighly specific radioimmunoassay for human insulin based on immune exclusion of all insulin precursors.
We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed, respectively, against the B10 region, the junction between A chain and C-peptide, and the junction between B chain and C-peptide), their affinity constant (approximately 10(10) L/mol), and their...
متن کاملLaboratory application of the dextran-coated-charcoal radioimmunoassay of insulin.
A modification of the method for radioimmunoassayof insulin is described, which achievessatisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution fo...
متن کاملRadioimmunoassay for insulin-like growth factor II (IGF-II).
Insulin-like growth factor II (IGF-II) levels in human plasma were measured in physiological and pathological conditions by radioimmunoassay (RIA) with biosynthetic IGF-II. This RIA was specific for IGF-II and cross-reactivity with IGF-I was 1%. The sensitivity was 15 pg/tube with 50% displacement at 50 pg/tube. The intra- and inter-assay coefficients of variation for IGF-II were 6.3 and 9.3%, ...
متن کاملStudies on nonprecipitating insulin antibody. Radioimmunoassay of insulin binding capacity and its relationship to insulin neutralizing activity and ability to evoke cutaneous anaphylactic reaction.
Skom and Talmage have demonstrated a non-precipitating antibody against insulin in the sera of insulin-treated diabetics. But their method of determining the antibody is not quantitative. In the present study, a titration of the insulin binding activity of anti-insulin was performed more quantitatively using 1311-labeled insulin and antiguinea pig r-globulin rabbit sera under the condition of i...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1970
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.19.8_371